ATP6V1A Antibody (GT3846) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13540

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Porcine (96%), Rhesus Macaque (99%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT3846

Format

Azide and BSA Free
View all ATP6V1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ATP6V1A Antibody (GT3846) - Azide and BSA Free

Western Blot: ATP6V1A Antibody (GT3846) [NBP3-13540]

Western Blot: ATP6V1A Antibody (GT3846) [NBP3-13540]

Western Blot: ATP6V1A Antibody (GT3846) [NBP3-13540] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT3846] (NBP3-13540) diluted at 1:500.
Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT3846) [NBP3-13540]

Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT3846) [NBP3-13540]

Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT3846) [NBP3-13540] - Rat liver. ATP6V1A antibody [GT3846] (NBP3-13540) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: ATP6V1A Antibody (GT3846) [NBP3-13540]

Flow Cytometry: ATP6V1A Antibody (GT3846) [NBP3-13540]

Flow Cytometry: ATP6V1A Antibody (GT3846) [NBP3-13540] - ATP6V1A antibody [GT3846] (NBP3-13540) detects ATP6V1A protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: ATP6V1A antibody [GT3846] (NBP3-13540) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
Western Blot: ATP6V1A Antibody (GT3846) [NBP3-13540]

Western Blot: ATP6V1A Antibody (GT3846) [NBP3-13540]

Western Blot: ATP6V1A Antibody (GT3846) [NBP3-13540] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT3846] (NBP3-13540) diluted at 1:1000.
Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT3846) [NBP3-13540]

Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT3846) [NBP3-13540]

Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT3846) [NBP3-13540] - Mouse brain. ATP6V1A antibody [GT3846] (NBP3-13540) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for ATP6V1A Antibody (GT3846) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1A

ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Long Name

V-type proton ATPase catalytic subunit A

Alternate Names

ATP6A1, ATP6V1A1, EC 7.1.2.2, V-ATPase subunit A, VPP2

Gene Symbol

ATP6V1A

Additional ATP6V1A Products

Product Documents for ATP6V1A Antibody (GT3846) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATP6V1A Antibody (GT3846) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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