B220/CD45R Antibody (PTPRC/1783R)
Novus Biologicals | Catalog # NBP2-53303
Recombinant Monoclonal Antibody
![Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.rndsystems.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Western-Blot-NBP2-53303-img0006.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PTPRC/1783R
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Product Specifications
Immunogen
Recombinant human full-length B220/CD45R protein (Uniprot: P08575 )
Localization
Cell Surface and Cytoplasmic
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for B220/CD45R Antibody (PTPRC/1783R)
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).![Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.rndsystems.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunocytochemistry-Immunofluorescence-NBP2-53303-img0003.jpg "Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.rndsystems.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0004.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.rndsystems.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0005.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of PFA-fixed Raji cells. B220/CD45R antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.rndsystems.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0001.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RB Recombinant Rabbit Monoclonal Antibody (PTPRC/1783R).![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]](https://resources.rndsystems.com/images/products/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0002.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]")
Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]
Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).Applications for B220/CD45R Antibody (PTPRC/1783R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-53303 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: B220/CD45R
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (PTPRC/1783R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for B220/CD45R Antibody (PTPRC/1783R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for B220/CD45R Antibody (PTPRC/1783R)
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Application: Immunohistochemistry-ParaffinSample Tested: FFPE and human lung cancerSpecies: HumanVerified Customer | Posted 10/13/202010X
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
