B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80588
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, COMET
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 62N3G8
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27440778).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Detection of B7-1/CD80 in Human Hodgkin's Lymphoma via seqIF™ staining on COMET™
B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Mouse Anti-Human B7-1/CD80, Monoclonal Antibody at 20ug/mL at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.![Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]](https://resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Western-Blot-NBP2-80588-img0002.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]")
Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]
Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.![Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]](https://resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80588-img0003.jpg "Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]")
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining. Image from the standard format of this antibody.![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]](https://resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80588-img0004.jpg "Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]")
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified customer review. Image from the standard format of this antibody.![Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]](https://resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Western-Blot-NBP2-80588-img0001.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]")
Western Blot: B7-1/CD80 Antibody (62N3G8)Azide and BSA Free [NBP2-80588]
Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis in human Raji lymphoma cells. Image from the standard format of this antibody.![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]](https://resources.rndsystems.com/images/products/B7-1-CD80-Antibody-62N3G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80588-img0005.jpg "Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]")
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588]
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Raji lymphoma cells. Image from verified customer review. Image from the standard format of this antibody.Applications for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000. Use reported by customer review
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
reported by customer review
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: B7-1/CD80
Alternate Names
B71, CD80
Gene Symbol
CD80
Additional B7-1/CD80 Products
Product Documents for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free and earn rewards!
Have you used B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
