BAF180/PB1 Antibody (BL-39-2C3)

Novus Biologicals | Catalog # NBP2-76400

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-39-2C3
View all BAF180/PB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein representing residues 1-293 of human Polybromo 1 (NP_060783.3 )

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF180/PB1 Antibody (BL-39-2C3)

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from Ba/F3 and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 10 seconds.
BAF180/PB1 Antibody (BL-39-2C3)

Simple Western: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] -

Simple Western: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of mouse PBRM1 by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (NBP2-76400) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally..
Immunocytochemistry/ Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunocytochemistry/ Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.
Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti- PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunocytochemistry/ Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunocytochemistry/ Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of human PBRM1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (NBP2-76400).
Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400]

Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of human PBRM1 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-3-2F2] (NBP2-76400) and rabbit anti-PBRM1 (NB100-1559 and NB100-1761).
BAF180/PB1 Antibody (BL-39-2C3)

Flow Cytometry: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] -

Flow Cytometry: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinantmonoclonal [BL-39-2C3] or isotypecontrol (unshaded). Secondary: DyLight® 488-conjugatedgoat anti-rabbit IgG.

Applications for BAF180/PB1 Antibody (BL-39-2C3)

Application
Recommended Usage

Flow Cytometry

Fixed and permeabilized cells were stained with 0.8 µl per 1 x 10^6

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

5 µl/mg lysate

Simple Western

1:50-1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
See Simple Western Antibody Database for Simple Western validation: Tested in 3T3 [0.08 mg/mL], separated by Size, antibody dilution of 1:250, 1:50, apparent MW was 195-196 kDa

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: BAF180/PB1

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PBRM1 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PBRM1 has been proposed to function as a tumor suppressor protein. Recently, PBRM1 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21.

Long Name

Protein polybromo-1

Alternate Names

BAF180, hPB1, PB1, PBRM1, Polybromo-1D

Gene Symbol

PBRM1

Additional BAF180/PB1 Products

Product Documents for BAF180/PB1 Antibody (BL-39-2C3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BAF180/PB1 Antibody (BL-39-2C3)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BAF180/PB1 Antibody (BL-39-2C3)

There are currently no reviews for this product. Be the first to review BAF180/PB1 Antibody (BL-39-2C3) and earn rewards!

Have you used BAF180/PB1 Antibody (BL-39-2C3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies