BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free
Novus Biologicals | Catalog # NBP1-97622
![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]](https://resources.rndsystems.com/images/products/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgM Clone # Buffy-2
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human soluble BAFF (aa 83-285).
Reactivity Notes
There is a weak corss-reactivity to Mouse.
Specificity
Recognizes membrane-bound and soluble human and mouse (weak) BAFF.
Clonality
Monoclonal
Host
Rat
Isotype
IgM
Scientific Data Images for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free
Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]
Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]](https://resources.rndsystems.com/images/products/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]")
Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]
Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.Applications for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Reviewed Applications
Read 1 review rated 5 using NBP1-97622 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Thiophilic Chromatography
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BAFF/BLyS/TNFSF13B
Long Name
B cell Activating Factor
Alternate Names
BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4
Gene Symbol
TNFSF13B
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free
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Customer Reviews for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free (1)
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1 Customer Rating
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Application: Immunohistochemistry-ParaffinSample Tested: human skinSpecies: HumanVerified Customer | Posted 01/21/2020Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained.Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
