Bcl-2 Antibody (100/D5)

Novus Biologicals | Catalog # NBP2-15200

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Rat (Negative)

Cited:

Human, Mouse, Rat

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, COMET

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Flow Cytometry Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 100/D5
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein. (Uniprot: P10415 )

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Marker

Apoptosis & Follicular Lymphoma Marker

Specificity

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33315)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Bcl-2 Antibody (100/D5)

Bcl-2 Antibody (100/D5) Detection of BCL2 in Human Non-Hodgkin’s Lymphoma via seqIF™ staining on COMET™

Detection of BCL2 in Human Non-Hodgkin’s Lymphoma via seqIF™ staining on COMET™

BCL2 was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkin’s Lymphoma using Mouse Anti-Human BCL2, Monoclonal Antibody (Catalog #NBP2-15200) at a 1:500 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). TTissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200]

Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200]

Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (100/D5).
Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200] - Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Ab (100/D5). Note nuclear membrane & cytoplasmic staining.
Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200]

Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200]

Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200] - Intracellular staining of Bcl-2 in fixed and permeabilized Jurkat cells using Bcl-2 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.
Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200]

Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200]

Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - analysis of Bcl-2 in human skin using Bcl-2 antibody at 2 ug/ml.

Applications for Bcl-2 Antibody (100/D5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Use in IP reported in scientific literature (PMID:35304128) Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined..

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-2

Bcl-2 (Bcl2) is the founding member of the Bcl-2 family. Family members have pivotal roles in regulating apoptosis or death signaling pathways through their control of mitochondrial permeability and cytochrome release (reviewed in Anvekar, 2011; Martinou and Youle, 2011). Bcl-2 derives its name from B cell lymphoma 2 where was first found to be highly expressed in follicular lymphomas with 14;18 reciprocal translocations. There are two isoforms, alpha and beta, generated by alternative splicing and differing in their carboxy termini. Human Bcl-2 alpha is a 239 amino acid (aa) protein and human Bcl-2 beta is a 205 aa protein. The 100/D5 antibody [also known as clone Bcl-2/100 (Kren, 2004, Kaur, 2004)] recognizes both Bcl-2 isoforms (Pezzela et al, 1990).
Bcl-2 is over expressed in neoplastic germinal centers of a majority of follicular lymphomas, whereas the normal or hyperplastic germinal centers are primarily negative for Bcl-2 expression. Upregulation has also been described in a number of other types of tumors. Bcl-2 expression is often considered to be a marker of cell death status, and over or high expression has often been tied to anti-apoptotic states, or resistance to death. However, the actual status of vulnerability to death can depend on the balance of other Bcl-2 family members present, their interaction with one another, as well as other factors.
This antibody has been extensively used to monitor the status of Bcl-2 expression in a myriad of normal and cancerous tissue types, developmental stages, as well as in cultured cells.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

UniProt

P10415

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (100/D5)

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody (100/D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-2 Antibody (100/D5)

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Protocols

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FAQs

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