Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80607

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Radioimmunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HL 1499

Format

Azide and BSA Free
View all Calcium-sensing R/CaSR Primary Antibodies »

Product Specifications

Immunogen

N-terminal region of human Calcium Sensing Receptor. [UniProt# P41180]

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free

Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid
Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells. Image from the standard format of this antibody.
Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue. Image from the standard format of this antibody.
Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607]

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.
Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.
Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499)Azide and BSA Free [NBP2-80607]

Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

5 - 10 ug

Simple Western

1:200

Western Blot

1:50 - 1:200
Application Notes
In Western blot transfected samples were tested, where a band was seen ~130 kDa and a large smear at the top of the gel.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:200. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calcium-sensing R/CaSR

Calcium Sensing Receptor (CaSR) was the first GPCR identified whose main physiological ligand is an ion and it has the ability to sense extracellular concentration of Ca2+. CaSR's activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and it is capable of interaction with VCP, RNF19A (execute CASR's ubiquitination) and ARRB1. CaSR is expressed primarily in chief cells of parathyroid glands where, when activated by high Ca2+ concentrations, it decreases PTH release (calcium-retaining hormone) to maintain Ca2 +. Alternatively, under hypocalcemia conditions, CaSR is inactive and PTH is released which restores normocalcemia by acting on kidney as well as intestine to increase Ca2 + absorption, and on bone, to mobilise skeletal Ca2 +. CaSR is also expressed in other tissues which do not play an obvious role in Ca2 + homeostasis (breast, blood vessels, liver, and placenta) and altered CaSR expression and/or activity have been found associated with parathyroid glands disorders, osteoporosis, vascular calcification and cancer. CaSR is implicated in regulation of diverse processes that includes hormone secretion, gene expression, ion channel activity, modulation of inflammation, proliferation, differentiation, and apoptosis, depending on cell type, and therefore, represents a key molecule in physiology. Defects in CaSR have been linked to familial hypocalciuric hypercalcemia type 1 (FHH), neonatal severe primary hyperparathyroidism (NSHPT), familial isolated hypoparathyroidism (FIH) and epilepsy, idiopathic generalized type 8 (EIG8).

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Gene Symbol

CASR

Additional Calcium-sensing R/CaSR Products

Product Documents for Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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