Carbonic Anhydrase I/CA1 Antibody (26E3) - BSA Free

Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] -

Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Image of Carbonic Anhydrase I/CA1 Antibody (26E3) diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.

Western Blot: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] -

Western Blot: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Positive Western Blot detected in: K562 whole cell lysate, U937 whole cell lysate.
All lanes: Carbonic Anhydrase I/CA1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 28 kDa
Observed band size: 28 kDa

Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] -

Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Overlay Peak curve showing MCF-7 cells stained with Carbonic Anhydrase I/CA1 Antibody (26E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.