Carbonic Anhydrase IX/CA9 Antibody [HRP]

Novus Biologicals | Catalog # NB100-417H

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Plant

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, ELISA, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Proximity Ligation Assay, Gel Super Shift Assays, Microarray

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all Carbonic Anhydrase IX/CA9 Primary Antibodies »

Product Specifications

Immunogen

This Carbonic Anhydrase IX/CA9 Antibody was made from a synthetic peptide from the C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt# Q16790].

Localization

Membrane.

Marker

Hypoxia Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase IX/CA9 Antibody [HRP]

Western Blot Detection of Carbonic Anhydrase IX/CA9 in Transfected HEK Cell Lysate Using HRP Conjugated Antibody

Western Blot Detection of Carbonic Anhydrase IX/CA9 in Transfected HEK Cell Lysate Using HRP Conjugated Antibody

Detection of carbonic anhydrase IX in transfected HEK cell lysate using NB 100-417. Rabbit IgG was used as a negative control.

Applications for Carbonic Anhydrase IX/CA9 Antibody [HRP]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Dual RNAscope ISH-IHC

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Gel Super Shift Assays

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Microarray

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Carbonic Anhydrase IX/CA9

Carbonic anhydrase alpha, isozyme IX, belongs to a family of zinc-containing metalloproteins which hydrate carbon dioxide to generate bicarbonate ions and protons (1). This main catalytic function allows carbonic anhydrase IX to participate in cellular pH regulation. The large family of carbonic anhydrase metalloproteins includes three major classes which have been identified based on sequence and structure analysis. The alpha class is a monomer found in mammals. The beta class may occur as a dimer, tetramer, hexamer or octamer and is found in plants, algae, and bacteria. Lastly, the gamma class is a trimer found in bacteria and represents the most ancient carbonic anhydrase. These three classes of carbonic anhydrase enzymes lack sequence or structural similarities, but all share a conserved active site zinc atom (1).

Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).

References

1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200

2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9

3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977

4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0

5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394

6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725

Alternate Names

CA9, G250, MN, RCC

Gene Symbol

CA9

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Carbonic Anhydrase IX/CA9 Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Carbonic Anhydrase IX/CA9 Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Carbonic Anhydrase IX/CA9 Antibody [HRP]

Showing  1 - 2 的 2 FAQs Showing All

  • Q: I would like to purchase your Carbonic Anhydrase IX Antibody [Biotin] 0.1ml for use in IHC-p. However, I would like to know before what sort of positive and negative controls you recommend.

    A:

    Our carbonic anhydrase IX antibody with catalogue number NB100-417B is covered by the Novus Guarantee for detection of the protein in human, mouse, rat and canine samples by IHC-P. As you know, carbonic anhydrase IX is expressed primarily in carcinoma cell lines. We sell a carbonic anhydrase IX tissue slide pack, with catalogue number S164W0, in which the tissue was derived from a renal cell carcinoma. Expression of carbonic anhydrase IX is restricted to very few normal tissues and we sell a range of normal tissue slides, which you can see at the following link: Normal Human Tissue Slides. We also sell a blocking peptide specific to this antibody, which has catalogue number NB100-417PEP.

  • Q: May I ask you a technical point about a western blotting using antibody NB100-417H? What is the second band observed round 25 kDa? Do you often see it? Material is total protein extracted from placenta villi.

    A: We did not see this band in our lab tests, however these were performed on HEK cell lysates. This antibody was generated using a synthetic peptide to the C-terminal of CA IX and the C-terminal does share sequence similarity to some truncated forms of CA IX and other CAs. Perhaps this is what you are detecting?

  • Q: I would like to purchase your Carbonic Anhydrase IX Antibody [Biotin] 0.1ml for use in IHC-p. However, I would like to know before what sort of positive and negative controls you recommend.

    A:

    Our carbonic anhydrase IX antibody with catalogue number NB100-417B is covered by the Novus Guarantee for detection of the protein in human, mouse, rat and canine samples by IHC-P. As you know, carbonic anhydrase IX is expressed primarily in carcinoma cell lines. We sell a carbonic anhydrase IX tissue slide pack, with catalogue number S164W0, in which the tissue was derived from a renal cell carcinoma. Expression of carbonic anhydrase IX is restricted to very few normal tissues and we sell a range of normal tissue slides, which you can see at the following link: Normal Human Tissue Slides. We also sell a blocking peptide specific to this antibody, which has catalogue number NB100-417PEP.

  • Q: May I ask you a technical point about a western blotting using antibody NB100-417H? What is the second band observed round 25 kDa? Do you often see it? Material is total protein extracted from placenta villi.

    A: We did not see this band in our lab tests, however these were performed on HEK cell lysates. This antibody was generated using a synthetic peptide to the C-terminal of CA IX and the C-terminal does share sequence similarity to some truncated forms of CA IX and other CAs. Perhaps this is what you are detecting?

Showing  1 - 2 的 2 FAQs Showing All
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