Caspase-8 Antibody (3B10) - BSA Free
Novus Biologicals | Catalog # NBP2-80098
![Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]](https://beta-resources.rndsystems.com/images/products/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0004.jpg "Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]")
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 kappa Clone # 3B10
Format
BSA Free
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Product Specifications
Immunogen
Recombinant mouse caspase-8 (p20 subunit).
Specificity
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1 kappa
Description
Novus Biologicals Rat Caspase-8 Antibody (3B10) - BSA Free (NBP2-80098) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Caspase-8 Antibody (3B10) - BSA Free
Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]
Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.![Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098]](https://beta-resources.rndsystems.com/images/products/Caspase-8-Antibody-3B10-BSA-Free-Flow-Cytometry-NBP2-80098-img0001.jpg "Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098]")
Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098]
Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (3B10) (Prod. No. NBP2-80098)![Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]](https://beta-resources.rndsystems.com/images/products/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0002.jpg "Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]")
Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]
Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.![Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]](https://beta-resources.rndsystems.com/images/products/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0003.jpg "Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]")
Western Blot: Caspase-8 Antibody (3B10)BSA Free [NBP2-80098]
Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.Applications for Caspase-8 Antibody (3B10) - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-8
Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.
Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.
Alternate Names
CASP8, Caspase8, Mch5
Gene Symbol
CASP8
Additional Caspase-8 Products
Product Documents for Caspase-8 Antibody (3B10) - BSA Free
Product Specific Notices for Caspase-8 Antibody (3B10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Caspase-8 Antibody (3B10) - BSA Free
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Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
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Associated Pathways
