CBX4 Antibody (6C5G3) - BSA Free

Novus Biologicals | Catalog # NBP2-52432

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6C5G3

Format

BSA Free
View all CBX4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CBX4 (AA: 397-514) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBX4 Antibody (6C5G3) - BSA Free

Western Blot: CBX4 Antibody (6C5G3)BSA Free [NBP2-52432]

Western Blot: CBX4 Antibody (6C5G3)BSA Free [NBP2-52432]

Western Blot: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis using CBX4 mAb against human CBX4 (AA: 397-514) recombinant protein. (Expected MW is 43.3 kDa)
Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis of rectum cancer tissues using CBX4 mouse mAb with DAB staining.
Flow Cytometry: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

Flow Cytometry: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

Flow Cytometry: CBX4 Antibody (6C5G3) [NBP2-52432] - Flow cytometric analysis of Hela cells using CBX4 mouse mAb (green) and negative control (red).
Western Blot: CBX4 Antibody (6C5G3)BSA Free [NBP2-52432]

Western Blot: CBX4 Antibody (6C5G3)BSA Free [NBP2-52432]

Western Blot: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis using CBX4 mAb against HEK293 (1) and CBX4 (AA: 397-514)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis of cervical cancer tissues using CBX4 mouse mAb with DAB staining.
ELISA: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

ELISA: CBX4 Antibody (6C5G3) - BSA Free [NBP2-52432]

ELISA: CBX4 Antibody (6C5G3) [NBP2-52432] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for CBX4 Antibody (6C5G3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBX4

PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.

Alternate Names

chromobox homolog 4, chromobox homolog 4 (Drosophila Pc class), Chromobox protein homolog 4, Drosophila), E3 SUMO-protein ligase CBX4, hPC2, NS5ATP1-binding protein 16, Pc class 2 homolog, Pc2, Polycomb 2 homolog

Gene Symbol

CBX4

Additional CBX4 Products

Product Documents for CBX4 Antibody (6C5G3) - BSA Free

Certificate of Analysis

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Product Specific Notices for CBX4 Antibody (6C5G3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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