CD11c Antibody (CA11.6A1) - BSA Free
Novus Biologicals | Catalog # NB100-65291
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Key Product Details
Species Reactivity
Canine
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CA11.6A1
Format
BSA Free
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Product Specifications
Immunogen
Made to Dog CD11c
Reactivity Notes
Predicted cross-reactivities: Hooded Seal, Raccoon
Marker
Dendritic Cell Marker
Specificity
Recognizes the canine CD11c cell surface antigen, a member of the alpha integrin family. Canine CD11c is expressed by monocytes, granulocytes and by dendritic cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse CD11c Antibody (CA11.6A1) - BSA Free (NB100-65291) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD11c Antibody (CA11.6A1) - BSA Free
Flow Cytometry: CD11c Antibody (CA11.6A1) [NB100-65291]
Flow Cytometry: CD11c Antibody (CA11.6A1) [NB100-65291] - Figure A. PE conjugated Mouse anti Canine CD45 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD45 and purified Mouse anti Canine CD11c detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on monocytes.Applications for CD11c Antibody (CA11.6A1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Reviewed Applications
Read 1 review rated 2 using NB100-65291 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD11c
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Gene Symbol
ITGAX
Additional CD11c Products
Product Documents for CD11c Antibody (CA11.6A1) - BSA Free
Product Specific Notices for CD11c Antibody (CA11.6A1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CD11c Antibody (CA11.6A1) - BSA Free (1)
2 out of 5
1 Customer Rating
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Application: Immunohistochemistry-FrozenSample Tested: Optic nerveSpecies: FelineVerified Customer | Posted 12/08/2017NB100-65291 was tested at 1:10-400 dilution as recommended, with incubation for overnight at 4C degree, and Alexa488 conjugated antibody was used. Image was captured with epifluorescent microscope.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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