Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2H7

Format

BSA Free
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

Human tonsillar B cells

Specificity

The antibody MFG-06 reacts with a 40-45 kDa glycoprotein in human milk fat globule membrane preparation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD20 Antibody (2H7) - BSA Free (NBP2-62206) is a monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD20 Antibody (2H7) - BSA Free

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) FITC.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM7.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM5.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Alexa Fluor(R) 700.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC-CyTM7.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Pacific BlueTM.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP-CyTM5.5.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE-DyLigt(R) 594.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP.
Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC.
CD20 Antibody (2H7) Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206]

Separation of human CD20 positive lymphocytes(red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of peripheral whole blood stained using anti-human CD20 (2H7) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).

Applications for CD20 Antibody (2H7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD20

CD20 is a non-glycosylated phosphoprotein that is expressed on the surface of normal and malignant B cells and functions in mediating calcium transport and B cell differentiation (1,2). CD20 is encoded by the membrane-spanning 4-domain family A member 1 (MS4A1) gene and, in humans, is located on chromosome 11q12 (1). The CD20 protein is 297 amino acids (aa) in length with a theoretical molecular weight (MW) of 33 kDa (1,2). Structurally, the CD20 protein has four membrane-spanning domains, two extracellular loop domains, and intracellular N- and C-terminal domains (1,2). CD20 is expressed at specific stages of B cell maturation including pre-B cells, mature naive and activated B cells, and memory B cells, but is absent from plasmablasts and plasma cells (1,3,4). Expression of CD20 is often increased on malignant B cells associated with various B cell disorders such as chronic lymphocytic leukemia (CLL), multiple sclerosis (MS), and rheumatoid arthritis (2-4). Anti-CD20 monoclonal antibody (mAb)-based therapies have become an appealing target for treating these immune-related disorders and cancers (1-5). Rituximab, a chimeric mAb, was the first FDA approved CD20 monoclonal antibody for the treatment of non-Hodgkin's lymphoma that is now commonly used to treat MS (2,3). Since its initial approval in 1997, several other chimeric and humanized anti-CD20 mAbs have been developed including Ofatumumab, Ublituximab, and Obinutuzumab (1-5). B cell depletion via CD20 mAbs can occur under different mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), complement-dependent cytotoxicity (CDC), and direct induction of apoptosis (1-4). Many ongoing studies are focused on combination therapies with CD20 mAbs as an addition to chemotherapy, B cell receptor (BCR) signaling inhibitors, or BH3 mimetics (1,2,4). Additionally, the effects of bispecific antibodies and CD20 chimeric antigen receptor (CAR) T cell therapies are under investigation for the treatment of B cell malignancies (4,5).

References

1. Pavlasova G, Mraz M. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020; 105(6):1494-1506. https://doi.org/10.3324/haematol.2019.243543

2. Payandeh Z, Bahrami AA, Hoseinpoor R, et al. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomed Pharmacother. 2019; 109:2415-2426. https://doi.org/10.1016/j.biopha.2018.11.121

3. Margoni M, Preziosa P, Filippi M, Rocca MA. Anti-CD20 therapies for multiple sclerosis: current status and future perspectives. J Neurol. 2022; 269(3):1316-1334. https://doi.org/10.1007/s00415-021-10744-x

4. Klein C, Jamois C, Nielsen T. Anti-CD20 treatment for B-cell malignancies: current status and future directions. Expert Opin Biol Ther. 2021; 21(2):161-181. https://doi.org/10.1080/14712598.2020.1822318

5. Sharman JP. Targeting CD20: teaching an old dog new tricks. Hematology Am Soc Hematol Educ Program. 2019; 2019(1):273-278. https://doi.org/10.1182/hematology.2019000031

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for CD20 Antibody (2H7) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD20 Antibody (2H7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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