CD81 Antibody (1D6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80660

Clone 1D6 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Mouse, Goat, Primate, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D6

Format

Azide and BSA Free
View all CD81 Primary Antibodies »

Product Specifications

Immunogen

Aggregated OCI-Ly8 human B cell line

Reactivity Notes

Chimpanzee

Localization

Membrane; Multi-pass membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD81 Antibody (1D6) - Azide and BSA Free

Western Blot: CD81 Antibody (1D6)Azide and BSA Free [NBP2-80660]

Western Blot: CD81 Antibody (1D6)Azide and BSA Free [NBP2-80660]

Western Blot: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep36502), licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - The TAPA1 Antibody (1D6) was tested in A431 cells at a 1:10 dilution against DyLight 488 (green). Actin was counterstained against Phalloidin 568 (red) and cells were mounted in DAPI Flouromount (blue). Image from the standard format of this antibody.
Immunohistochemistry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Immunohistochemistry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Immunohistochemistry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Staining of human peripheral blood lymphocytes. Image from the standard format of this antibody.
Western Blot: CD81 Antibody (1D6)Azide and BSA Free [NBP2-80660]

Western Blot: CD81 Antibody (1D6)Azide and BSA Free [NBP2-80660]

Western Blot: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL. Image from the standard format of this antibody.
Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were directly conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.
Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660]

Flow Cytometry: CD81 Antibody (1D6) - Azide and BSA Free [NBP2-80660] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used. Image from the standard format of this antibody.

Applications for CD81 Antibody (1D6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.2 - 1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1 - 2 ug/ml
Application Notes
NB100-65805 is a potent TAPA1/CD81 reagent, it induces homotypic adhesion and has powerful anti-proliferative effects. For Flow Cytometry: Use 10 uL of the suggested working dilution to label 106 cells in 100 uL. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD81

TAPA1, also known as CD81, is found in the plasma membrane in lymphocytes and is believed to play an important role in the regulation of lymphoma cell growth. This protein is primarily found on B and T cells (lymphocytes) in humans. TAPA1 appears to be involved in a variety of cellular processes including activation, proliferation and differentiation. It is believed to act as a viral receptor for HCV and may play a crucial role in cell signaling from its place embedded in the plasma membrane. Mutations affecting CD81 can cause immunodeficiency- a disease that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections due to a lack of an immune response.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (1D6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD81 Antibody (1D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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