Coronin-1a Antibody

Novus Biologicals | Catalog # NB100-1298

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Coronin-1a Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRLDRLEETVQAK corresponding to C-Terminus according to NP_009005.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine, Mouse.

Localization

In noninfected macrophages, associated with the cortical microtubule network; in mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly depend

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Coronin-1a Antibody

Western Blot: Coronin-1a Antibody [NB100-1298]

Western Blot: Coronin-1a Antibody [NB100-1298]

Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298]

Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298]

Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Coronin-1a Antibody [NB100-1298]

Flow Cytometry: Coronin-1a Antibody [NB100-1298]

Flow Cytometry: Coronin-1a Antibody [NB100-1298] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Coronin-1a Antibody [NB100-1298]

Western Blot: Coronin-1a Antibody [NB100-1298]

Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Daudi (A), MOLT4 (B) and (1ug/ml) of Jurkat (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298]

Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298]

Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Coronin-1a Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/mL

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Coronin-1a

Coronins are actin-binding proteins in eucaryotic cells. The prototype of coronin were found in Dictyostelium discoideum. The protein is involved in many cell processes, e.g. phagocytosis, cytokinesis and migration.

Alternate Names

CLIPINA, Clipin-A, CORO1, coronin, actin binding protein, 1A, coronin, actin-binding protein, 1A, coronin-1, coronin-1A, Coronin-like protein A, Coronin-like protein p57, FLJ41407, HCORO1, MGC117380, p57, TACOCLABP, Tryptophan aspartate-containing coat protein

Entrez Gene IDs

11151 (Human); 12721 (Mouse); 155151 (Rat)

Gene Symbol

CORO1A

UniProt

NP_009005. (Human)

Additional Coronin-1a Products

Product Documents for Coronin-1a Antibody

Certificate of Analysis

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Product Specific Notices for Coronin-1a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Coronin-1a Antibody

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Protocols

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FAQs

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