COX-1 Antibody (SC68-05)

Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643]

Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643]

Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643]

Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-COX-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.