COX-2 Antibody

Novus Biologicals | Catalog # NB100-868

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Plant

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NPTVLLKERSTEL corresponding to C-Terminus according to NP_000954.1.

Reactivity Notes

Expected from sequence similarity: Canine, Porcine, Bovine. Mouse reactivity reported in scientific literature (PMID: 24854039).

Localization

Microsomal membrane

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for COX-2 Antibody

Western Blot: COX-2 Antibody [NB100-868]

Western Blot: COX-2 Antibody [NB100-868]

Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NB100-868]

Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NB100-868]

Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NB100-868] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasm and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868]

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868]

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868] - 10x image of mouse lung tissue expressing cox-2. Secondary antibody: Alexa Fluor 546 (red), counterstain: DAPI (blue). Goat anti-mouse cox-2 immunofluorescence staining of paraffin embedded fixed mouse lung tissue sections. Antigen retrieval in citrate buffer (95C, 20 min) followed by permeabilization with 0.2% Triton X-100 for 20 min. Sections were blocked in 5% donkey serum for 1 hr at RT, and incubated with NB100-868 1:100 dilution in 3% donkey serum overnight at 4 C. Incubated with secondary donkey anti-goat AF546 at 1:500 dilution 1 hr at RT. Image submitted by a verified customer review.
Flow Cytometry: COX-2 Antibody [NB100-868]

Flow Cytometry: COX-2 Antibody [NB100-868]

Flow Cytometry: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line)
Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NB100-868]

Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NB100-868]

Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml, showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue)

Applications for COX-2 Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1-0.3ug/ml
Application Notes
Western blot: Approx 75-80 kDa band observed in lysates of cell lines A549 and Daudi (predicted MW of 69 kDa according to NP_000954.1). COX-2 antibody validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-868 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Entrez Gene IDs

5743 (Human)

Gene Symbol

PTGS2

UniProt

NP_000954. (Human)

Additional COX-2 Products

Product Documents for COX-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COX-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for COX-2 Antibody (1)

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Customer Images

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  • COX-2 Antibody
    Name: tyler beach
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult Mouse Lung Tissue
    Species: Mouse
    Verified Customer | Posted 05/09/2018
    10x image of mouse lung tissue expressing cox-2, seondary: alexafluor 546 (red), dapi (blue)
    Goat anti mouse cox 2 immunofluorescence staining of paraffin embedded fixed mouse lung tissue sections- Antigen retrieval in citrate buffer (95 C, 20 min) followed by permeabilization with 0.2% Triton x-100 for 20 min. Sections were blocked in 5% donkey serum for 1 hr at RT, and incubated with NB100-868 1:100 dilution in 3% donkey serum overnight at 4 C. Incubated with secondary donkey anti-goat AF546 at 1:500 dilution 1 hr at RT. Counter-stained with DAPI (blue)
    COX-2 Antibody NB100-868

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Protocols

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FAQs for COX-2 Antibody

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  • Q: I am a student researching COX-2 expression in felines and am looking for a suitable primary antibody to use in IHC. To your knowledge, have any of your COX-2 antibodies been used successfully with feline COX-2? If yes, I would be very interested in learning more. If not, would it be possible to run a sequence homology of feline COX-2 (GenBank ABM65700.1, accession EF036473.1) against your available COX-2 antibodies to determine the overlap and likelihood of cross-reactivity?

    A:

    We have not tested any of these antibodies for their reactivity in felines. However, we do have a number of antibodies that look like they may cross-react with feline. NBP1-85499 recognizes a region that is about 90-91% similar to feline COX2. NBP2-04015 recognizes a region in humans that is 94% similar to feline COX2. NB100-868 recognizes a region in humans at the far C-terminal 12 amino acids that is 100% identical to feline COX2. NBP1-19354 recognizes a region that is also about 95% similar to feline COX2. Only NBP1-85499 and NBP1-19354 have been tested for use in IHC. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.

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