CXCL8/IL-8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16958

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Predicted:

Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-8/CXCL8 Primary Antibodies »

Product Specifications

Immunogen

Full length human CXCL8/IL-8 Recombinant protein.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33504136). Sheep (81%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CXCL8/IL-8 Antibody - BSA Free (NBP2-16958) is a polyclonal antibody validated for use in IHC, WB and Flow. Anti-CXCL8/IL-8 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CXCL8/IL-8 Antibody - BSA Free

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-16958]

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-16958]

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-16958] - Human gastric cancer. CXCL8 / IL8 stained by CXCL8 / IL8 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
CXCL8/IL-8 Antibody

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] -

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8/IL-8 antibody (NBP2-16958) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
CXCL8/IL-8 Antibody

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] -

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8/IL-8 antibody (NBP2-16958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
CXCL8/IL-8 Antibody

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] -

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] - Human leukocyte (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8/IL-8 antibody (NBP2-16958) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CXCL8/IL-8 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-8/CXCL8

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq]

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Gene Symbol

CXCL8

UniProt

P10145

Additional IL-8/CXCL8 Products

Product Documents for CXCL8/IL-8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CXCL8/IL-8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CXCL8/IL-8 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCL8/IL-8 Antibody - BSA Free

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    • A: Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.
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