Cytokeratin 5/75 Antibody (SC62-04)

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human liver cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Western blot analysis on different lysates with NBP2-67586 at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: MCF7 cell lysate (negative) Lane 4: Mouse skin tissue lysate Lane 5: Rat skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 57 kDa Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67586 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.