dynactin 4 Antibody (3G9D7) - BSA Free

Novus Biologicals | Catalog # NBP2-37399

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G9D7

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human dynactin 4 (AA: 57-298) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for dynactin 4 Antibody (3G9D7) - BSA Free

Western Blot: dynactin 4 Antibody (3G9D7)BSA Free [NBP2-37399]

Western Blot: dynactin 4 Antibody (3G9D7)BSA Free [NBP2-37399]

Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Immunocytochemistry/ Immunofluorescence: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Immunocytochemistry/Immunofluorescence: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Immunohistochemistry: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Immunohistochemistry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 mouse mAb with DAB staining.
Flow Cytometry: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Flow Cytometry: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Flow Cytometry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Flow cytometric analysis of A549 cells using DCTN4 mouse mAb (green) and negative control (red).
Western Blot: dynactin 4 Antibody (3G9D7)BSA Free [NBP2-37399]

Western Blot: dynactin 4 Antibody (3G9D7)BSA Free [NBP2-37399]

Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against human DCTN4 recombinant protein. (Expected MW is 53.2 kDa)
Western Blot: dynactin 4 Antibody (3G9D7)BSA Free [NBP2-37399]

Western Blot: dynactin 4 Antibody (3G9D7)BSA Free [NBP2-37399]

Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against HEK293 (1) and DCTN4 (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Immunohistochemistry: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

Immunohistochemistry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DCTN4 mouse mAb with DAB staining.
ELISA: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

ELISA: dynactin 4 Antibody (3G9D7) - BSA Free [NBP2-37399]

ELISA: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for dynactin 4 Antibody (3G9D7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: dynactin 4

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton

Alternate Names

dynactin 4 (p62), dynactin p62 subunit, dynactin subunit 4, Dynactin subunit p62

Gene Symbol

DCTN4

Additional dynactin 4 Products

Product Documents for dynactin 4 Antibody (3G9D7) - BSA Free

Certificate of Analysis

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Product Specific Notices for dynactin 4 Antibody (3G9D7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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