EIF5 Antibody (6D6H11) - BSA Free

Novus Biologicals | Catalog # NBP2-61762

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 6D6H11

Format

BSA Free
View all EIF5 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF5 Antibody (6D6H11) - BSA Free

Western Blot: EIF5 Antibody (6D6H11)BSA Free [NBP2-61762]

Western Blot: EIF5 Antibody (6D6H11)BSA Free [NBP2-61762]

Western Blot: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis using EIF5 mAb against human EIF5 (AA: 1-300) recombinant protein. (Expected MW is 60.2 kDa)
Immunocytochemistry/ Immunofluorescence: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

Immunocytochemistry/ Immunofluorescence: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

Immunocytochemistry/Immunofluorescence: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of Hela cells using EIF5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of rectum cancer tissues using EIF5 mouse mAb with DAB staining.
Western Blot: EIF5 Antibody (6D6H11)BSA Free [NBP2-61762]

Western Blot: EIF5 Antibody (6D6H11)BSA Free [NBP2-61762]

Western Blot: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis using EIF5 mAb against HEK293 (1) and EIF5 (AA: 1-300)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

Immunohistochemistry-Paraffin: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of bladder cancer tissues using EIF5 mouse mAb with DAB staining.
ELISA: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

ELISA: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

ELISA: EIF5 Antibody (6D6H11) [NBP2-61762] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

ELISA: EIF5 Antibody (6D6H11) - BSA Free [NBP2-61762]

ELISA: EIF5 Antibody (6D6H11) [NBP2-61762] - Analysis of Hela cells using EIF5 mouse mAb (green) and negative control (red).

Applications for EIF5 Antibody (6D6H11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF5

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).;

Alternate Names

eIF-5, EIF-5A, eukaryotic translation initiation factor 5

Gene Symbol

EIF5

Additional EIF5 Products

Product Documents for EIF5 Antibody (6D6H11) - BSA Free

Certificate of Analysis

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Product Specific Notices for EIF5 Antibody (6D6H11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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