ER beta/NR3A2 Antibody (ESR2/686)
Novus Biologicals | Catalog # NBP2-44366
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # ESR2/686
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Product Specifications
Immunogen
C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731)
Localization
Predominantly nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47797)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ER beta/NR3A2 Antibody (ESR2/686)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]](https://beta-resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0006.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]")
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]](https://beta-resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Flow-Cytometry-NBP2-44366-img0010.jpg "Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]")
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ESR2/686).![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]](https://beta-resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0003.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]")
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]](https://beta-resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0004.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]")
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]](https://beta-resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0005.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]")
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]](https://beta-resources.rndsystems.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Flow-Cytometry-NBP2-44366-img0009.jpg "Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]")
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Antibody (ESR2/686).Applications for ER beta/NR3A2 Antibody (ESR2/686)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ER beta/NR3A2
Additional ER beta/NR3A2 Products
Product Documents for ER beta/NR3A2 Antibody (ESR2/686)
Product Specific Notices for ER beta/NR3A2 Antibody (ESR2/686)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ER beta/NR3A2 Antibody (ESR2/686)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
