FoxO1/FKHR Antibody (3B6) - BSA Free

Name: FoxO1/FKHR Antibody (3B6) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-22190
Price: 199 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NBP2-22190

(1)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 3B6

Format

BSA Free

View all FoxO1/FKHR Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human FoxO1/FKHR expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

69.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FoxO1/FKHR Antibody (3B6) - BSA Free

Western Blot: FoxO1/FKHR Antibody (3B6)BSA Free [NBP2-22190]

Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (3B6) - BSA Free [NBP2-22190]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunofluorescence analysis of Hela cells using FOXO1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) - BSA Free [NBP2-22190]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1A mouse mAb with DAB staining.

Western Blot: FoxO1/FKHR Antibody (3B6)BSA Free [NBP2-22190]

Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)

Western Blot: FoxO1/FKHR Antibody (3B6)BSA Free [NBP2-22190]

Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.

ELISA: FoxO1/FKHR Antibody (3B6) - BSA Free [NBP2-22190]

ELISA: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

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Applications for FoxO1/FKHR Antibody (3B6) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Application Notes

WB reported from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-22190 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO1/FKHR

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Long Name

Forkhead Box O1

Alternate Names

FKH1, FKHR

Gene Symbol

FOXO1

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (3B6) - BSA Free

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FoxO1/FKHR Antibody (3B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Transcription Factors in the Akt Pathway

Customer Reviews for FoxO1/FKHR Antibody (3B6) - BSA Free (1)

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Name: Anonymous

Application: Western Blot

Sample Tested: Nuclear fraction of human breast cancer cell lines

Species: Human

Verified Customer | Posted 08/23/2015

FoxO1 expression in the fraction of human breast cancer cell lines. NBP2-22190