FOXO3 Antibody (24GB3120)

Novus Biologicals | Catalog # NBP3-50687

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB3120
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Product Specifications

Immunogen

A synthesized peptide derived from human FOXO3 (Uniprot: O43524)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FOXO3 Antibody (24GB3120)

FOXO3 Antibody (24GB3120)

Immunocytochemistry/ Immunofluorescence: FOXO3 Antibody (24GB3120)[NBP3-50687] -

Immunocytochemical staining of HepG2 cells with FOXO3 antibody (1:1,000). Nuclei were stained blue with DAPI; FOXO3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
FOXO3 Antibody (24GB3120)

Flow Cytometry: FOXO3 Antibody (24GB3120)[NBP3-50687] -

Flow cytometric analysis of FOXO3expression in HepG2 cells using FOXO3 antibody (1:2,000). Green, isotype control; red, Forkhead box O3.
FOXO3 Antibody (24GB3120)

Western Blot: FOXO3 Antibody (24GB3120)[NBP3-50687] -

Western blotting analysis using FOXO3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for FOXO3 Antibody (24GB3120)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxO3

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Gene Symbol

FOXO3

Additional FoxO3 Products

Product Documents for FOXO3 Antibody (24GB3120)

Certificate of Analysis

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Product Specific Notices for FOXO3 Antibody (24GB3120)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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