GABPB1 Antibody

Novus Biologicals | Catalog # NB100-60948

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GABPB1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2; NP_002032.2; NP_001307844.1.

Epitope

SSENSSKATDETG

Specificity

This antibody is expected to recognise isoform beta 1 (NP_005245.2), isoform gamma 1 (NP_002032.2), and beta-1 isoform 5 NP_001307844.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GABPB1 Antibody

Western Blot: GABPB1 Antibody [NB100-60948]

Western Blot: GABPB1 Antibody [NB100-60948]

Western Blot: GABPB1 Antibody [NB100-60948] - Using 0.3 ug/ml for the staining of HeLa cell lysate (A) + peptide (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.
GABPB1 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal GABPB1 Antibody [NB100-60948] -

Immunohistochemistry-Paraffin: Goat Polyclonal GABPB1 Antibody [NB100-60948] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear/nuclear speckle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
GABPB1 Antibody

Immunocytochemistry/Immunofluorescence: Goat Polyclonal GABPB1 Antibody [NB100-60948] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal GABPB1 Antibody [NB100-60948] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
GABPB1 Antibody

Flow Cytometry: Goat Polyclonal GABPB1 Antibody [NB100-60948] -

Flow Cytometry: Goat Polyclonal GABPB1 Antibody [NB100-60948] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948]

Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948]

Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948] - Using 3.75 ug/ml for the staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948]

Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948]

Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948] - Using 3.75 ug/ml for the staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GABPB1 Antibody

Application
Recommended Usage

ELISA

Detection limit 1:32000

Flow Cytometry

10 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx. 45+38 kDa band bands observed in HeLa lysates (calculated MW of 42.5 kDa band according to NP_005245.2, P_001307844.1, and of 38.1 kDa band according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABPB1

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (beta 1) represents the longest transcript and encodes the longest isoform (beta 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

E4TF1, E4TF1-47, E4TF1-53, E4TF1BBABPB2, GA binding protein transcription factor, beta subunit 1, GA binding protein transcription factor, beta subunit 2, GA-binding protein subunit beta-1, GABP subunit beta-1, GABPB-1, GABPB2, GABPB-2, GABPBGABP subunit beta-2, NRF2B1, NRF2B2, Nuclear respiratory factor 2, Transcription factor E4TF1-47, Transcription factor E4TF1-53

Entrez Gene IDs

126626 (Human)

Gene Symbol

GABPB1

UniProt

NP_005245.2 (Human), NP_002032.2 (Human), NP_001307844.1 (Human)

Additional GABPB1 Products

Product Documents for GABPB1 Antibody

Certificate of Analysis

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Product Specific Notices for GABPB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GABPB1 Antibody

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Protocols

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