GRB2 Antibody (JE49-73)

Name: GRB2 Antibody (JE49-73)
Brand: Novus Biologicals
SKU: NBP3-32403
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-32403

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE49-73 expressed in HEK293

View all GRB2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human GRB2 aa 1-100 / 217. (Uniprot: P62993)

Localization

Cytoplasm. Endosome. Golgi apparatus. Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRB2 Antibody (JE49-73)

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403]

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403] - Immunocytochemistry analysis of PC-12 cells labeling GRB2 with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403]

Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403] - Flow cytometric analysis of MCF7 cells labeling GRB2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32403, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403]

Western blot analysis on different lysates with NBP3-32403 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32403 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for GRB2 Antibody (JE49-73)

Application

Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRB2

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Long Name

Growth Factor Receptor-Bound Protein 2

Alternate Names

ASH, EGFRBP-GRB2, Grb3-3

Gene Symbol

GRB2

Additional GRB2 Products

Product Documents for GRB2 Antibody (JE49-73)

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Certificate of Analysis

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Product Specific Notices for GRB2 Antibody (JE49-73)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.