Human 2B4/CD244/SLAMF4 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF1039AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Agonist Activity, CyTOF-ready

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all 2B4/CD244/SLAMF4 Primary Antibodies »

Product Specifications

Specificity

Detects human 2B4/CD244/SLAMF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse 2B4, recombinant human (rh) SLAM, rhCD48, and rhCD84 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human 2B4/CD244/SLAMF4 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Agonist Activity

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

ELISA Capture (Matched Antibody Pair)

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 2B4/CD244/SLAMF4

2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1‑4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%‑24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma delta T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8‑10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7‑13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15).

References

  1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
  4. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
  5. Boles, K.S. et al. (1999) Tissue Antigens 54:27.
  6. Kubin, M.Z. et al. (1999) Eur. J. Immunol. 29:3466.
  7. Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
  8. Lee, K.M. et al. (2006) Blood 107:3181.
  9. Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
  10. Assarsson, E. et al. (2004) J. Immunol. 173:174.
  11. Bryceson, Y.T. et al. (2006) Blood 107:159.
  12. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
  13. McNerney, M.E. et al. (2005) Blood 106:1337.
  14. Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3870.
  15. Messmer, B. et al. (2006) J. Immunol. 176:4646.

Alternate Names

CD244, NAIL, NKR2B4, Nmrk, SLAMF4

Entrez Gene IDs

51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey)

Gene Symbol

CD244

UniProt

NP_057466

Additional 2B4/CD244/SLAMF4 Products

Product Documents for Human 2B4/CD244/SLAMF4 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 2B4/CD244/SLAMF4 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Customer Reviews

There are currently no reviews for this product. Be the first to review Human 2B4/CD244/SLAMF4 Alexa Fluor™ Plus 488‑conjugated Antibody and earn rewards!

Have you used Human 2B4/CD244/SLAMF4 Alexa Fluor™ Plus 488‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies