Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG1 Clone # 2416C
View all AdipoR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human AdipoR1 C-term sequences

Specificity

Detects human AdipoR1 in direct ELISAs. In direct ELISA, no cross-reactivity with human AdipoR2 is observed

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG1

Scientific Data Images for Human AdipoR1 Antibody

Detection of AdipoR1 antibody in HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP antibody by Flow Cytometry.

Detection of AdipoR1 in HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human AdipoR1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

AdipoR1 antibody in Human Placenta by Immunohistochemistry (IHC-P).

AdipoR1 in Human Placenta.

AdipoR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in decidual cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human AdipoR1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AdipoR1

Adiponectin receptor protein 1 (AdipoR1) is a 7 transmembrane receptor for globular and full-length adiponectin required for normal glucose and fat homeostasis and for maintaining a normal body weight.  AdipoR1 mediates increased AMP kinase and PPAR-alpha ligand activities well as fatty-acid oxidation and glucose uptake by adiponectin.  Genetic variability at the AdipoR1 locus is a strong determinant of coronary artery disease susceptibility in type 2 diabetes.  AdipoR1 is abundantly expressed in skeletal muscle.

Long Name

Adiponectin Receptor 1

Alternate Names

ACDCR1, CGI45, PAQR1, TESBP1A

Entrez Gene IDs

51094 (Human); 72674 (Mouse); 289036 (Rat)

Gene Symbol

ADIPOR1

Additional AdipoR1 Products

Product Documents for Human AdipoR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human AdipoR1 Antibody

For research use only

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Protocols

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FAQs

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