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Human BCMA/TNFRSF17 Antibody [Biotin]

R&D Systems | Catalog # BAF193

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Flow Cytometry

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all BCMA/TNFRSF17 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17
Met1-Ala54
Accession # Q6PE46

Specificity

Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BCMA/TNFRSF17 Antibody [Biotin]

Detection of BCMA/TNFRSF17 in U266 cells by Flow Cytometry

U266 cells were stained with Goat Anti-Human BCMA/TNFRSF17 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF193, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
Human BCMA / TNFRSF17 ELISA Standard Curve

Human BCMA / TNFRSF17 ELISA Standard Curve

Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) was serially diluted and captured by Goat Anti-Human BCMA/TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human BCMA/TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human BCMA/TNFRSF17 Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: See below

Western Blot

0.1 µg/mL
Sample: Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)

Human BCMA/TNFRSF17 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human BCMA/TNFRSF17 Antibody (Catalog # AF193)
  • Standard: Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (Catalog # 193-BC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using BAF193 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCMA/TNFRSF17

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

Q6PE46

Additional BCMA/TNFRSF17 Products

Product Documents for Human BCMA/TNFRSF17 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BCMA/TNFRSF17 Antibody [Biotin]

For research use only

Citations for Human BCMA/TNFRSF17 Antibody [Biotin]

Customer Reviews for Human BCMA/TNFRSF17 Antibody [Biotin] (3)

5 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Human BCMA/TNFRSF17 Biotinylated Antibody
    Name: Anonymous
    Application: Good pair with in-house monoclonal antibodies for a custom ELISA development
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/29/2025
    Human BCMA/TNFRSF17 Antibody [Biotin] BAF193
  • Human BCMA/TNFRSF17 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 04/05/2022
    Human BCMA/TNFRSF17 Antibody [Biotin] BAF193
  • Human/Primate BCMA/TNFRSF17 Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: U266 human myeloma cell line
    Species: Human
    Verified Customer | Posted 10/09/2020
    Human BCMA/TNFRSF17 Antibody [Biotin] BAF193

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