Human CCL5/RANTES Antibody (21445)

R&D Systems | Catalog # MAB278

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 21445
View all CCL5/RANTES Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL5/RANTES

Specificity

Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4,  recombinant mouse (rm) CCL3, and rmCCL4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL5/RANTES Antibody (21445)

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND50 is typically 0.1-0.4 µg/mL.
CCL5/RANTES antibody in Mouse Small Intestine by Immunohistochemistry (IHC-Fr).

CCL5/RANTES in Mouse Small Intestine.

CCL5/RANTES was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of CCL5/RANTES by Immunohistochemistry

Detection of CCL5/RANTES by Immunohistochemistry

CCR2 ligands are secreted but do not bind to HUVECs.a Culture medium collected from the upper and lower wells of transwells with HUVEC monolayers either not stimulated (NS) or stimulated with TNF-alpha + IFN-gamma were assayed for CCL2, CCL7, and CCL8 by ELISA (n = 3 separate experiments). Each symbol shows data from one experiment, with assays done in duplicate, and bars indicate means ± SEM. b Confocal microscopy images at × 40 magnification of permeabilized and non-permeabilized TNF-alpha + IFN-gamma -stimulated HUVECs immunostained for CCL2, CCL7, CCL8, CCL5, CCL20, and CXCL9 (green) and stained using phalloidin for polymerized actin (magenta) and DAPI for nuclei (blue). The scale bars indicate 10 µm. Images are representative of three experiments. c Biotinylated CCL5 (0.1 μM) and CCL2 (1 μM) were incubated with CHO-K1 cells and the heparan-sulfate deficient cell line, D-677. Chemokine binding was detected using streptavidin-phycoerythrin (PE) and flow cytometry. Data shown are representative of three separate experiments. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-025-57002-6), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCL5/RANTES by Flow Cytometry

Detection of CCL5/RANTES by Flow Cytometry

CCR2 ligands are secreted but do not bind to HUVECs.a Culture medium collected from the upper and lower wells of transwells with HUVEC monolayers either not stimulated (NS) or stimulated with TNF-alpha + IFN-gamma were assayed for CCL2, CCL7, and CCL8 by ELISA (n = 3 separate experiments). Each symbol shows data from one experiment, with assays done in duplicate, and bars indicate means ± SEM. b Confocal microscopy images at × 40 magnification of permeabilized and non-permeabilized TNF-alpha + IFN-gamma -stimulated HUVECs immunostained for CCL2, CCL7, CCL8, CCL5, CCL20, and CXCL9 (green) and stained using phalloidin for polymerized actin (magenta) and DAPI for nuclei (blue). The scale bars indicate 10 µm. Images are representative of three experiments. c Biotinylated CCL5 (0.1 μM) and CCL2 (1 μM) were incubated with CHO-K1 cells and the heparan-sulfate deficient cell line, D-677. Chemokine binding was detected using streptavidin-phycoerythrin (PE) and flow cytometry. Data shown are representative of three separate experiments. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-025-57002-6), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCL5/RANTES Antibody (21445)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human CCL5/RANTES transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)

Neutralization

Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB278 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for Human CCL5/RANTES Antibody (21445)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL5/RANTES Antibody (21445)

For research use only

Citations for Human CCL5/RANTES Antibody (21445)

Customer Reviews for Human CCL5/RANTES Antibody (21445) (2)

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  • Human CCL5/RANTES Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 09/18/2018
    Human CCL5/RANTES Antibody (21445) MAB278
  • Human CCL5/RANTES Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017

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