Human CD155/PVR Antibody

Catalog # Availability Size / Price Qty
MAB25301
MAB25301-SP
Human CD155/PVR Antibody in Data
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Product Details
Citations (6)
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Reviews (1)

Human CD155/PVR Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD155/PVR in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 300907
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD155/PVR
Gly27-Asn343
Accession # AAH15542
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD155/PVR (Catalog # 2530-CD)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD155/PVR in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of CD155/PVR in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD155/PVR in HUVEC Human Cells by Flow Cytometry. View Larger

Detection of CD155/PVR in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD155/PVR in Human PBMCs. View Larger

CD155/PVR in Human PBMCs. CD155/PVR was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6‑8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

References
  1. Takai, Y. et al. (2003) Cancer Sci. 94:655.
  2. Mendelsohn, C.L. et al. (1989) Cell 56:855.
  3. Koike, H. et al. (1990) EMBO J. 9:3217.
  4. Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
  5. Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
  6. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Lange, R. et al. (2001) Virology 285:218.
Long Name
Poliovirus Receptor
Entrez Gene IDs
5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names
CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

Product Datasheets

Citations for Human CD155/PVR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Trabectedin triggers direct and NK-mediated cytotoxicity in multiple myeloma
    Authors: M Cucè, ME Gallo Cant, MA Siciliano, C Riillo, D Caracciolo, F Scionti, N Staropoli, V Zuccalà, L Maltese, A Di Vito, K Grillone, V Barbieri, M Arbitrio, MT Di Martino, M Rossi, N Amodio, P Tagliaferr, P Tassone, C Botta
    J Hematol Oncol, 2019;12(1):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Binding of the Fap2 protein of Fusobacterium nucleatum to human inhibitory receptor TIGIT protects tumors from immune cell attack.
    Authors: Gur C, Ibrahim Y, Isaacson B, Yamin R, Abed J, Gamliel M, Enk J, Bar-On Y, Stanietsky-Kaynan N, Coppenhagen-Glazer S, Shussman N, Almogy G, Cuapio A, Hofer E, Mevorach D, Tabib A, Ortenberg R, Markel G, Miklic K, Jonjic S, Brennan C, Garrett W, Bachrach G, Mandelboim O
    Immunity, 2015;42(2):344-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  3. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
    Authors: Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
    Eur J Immunol, 2013;43(8):2138-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012;86(16):8693-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011;30(23):2622-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CD155/PVR Antibody
By Anonymous on 12/21/2018
Application: ELISA Sample Tested: Aortic smooth muscle cells Species: Human