Human CD19 Antibody (4G7-2E3) [Biotin]

Name: Human CD19 Antibody (4G7-2E3) [Biotin]
Brand: R&D Systems
SKU: FAB4867B
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # FAB4867B

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4G7-2E3

View all CD19 Primary Antibodies »

Product Specifications

Immunogen

Human CLL cells

Specificity

Detects human CD19 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CD19 Antibody (4G7-2E3) [Biotin]

Detection of CD19 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD19 Biotinylated Monoclonal Antibody (Catalog # FAB4867B) or (B) Mouse IgG₁Biotinylated Isotype Control (Catalog # IC002B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD19 Antibody (4G7-2E3) [Biotin]

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.