Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CD43 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD43
Met1-Arg253
Accession # P16150

Specificity

Detects human CD43 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CD43 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood lymphocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD43

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD43

CD43 (leukosialin, sialophorin, also Ly-48 in mouse) is a type I transmembrane (TM) sialated mucin that is found on leukocytes and tumor cells. It is synthesized as a 397 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 123 aa cytoplasmic tail that contains an NLS. The extracellular region has 12% proline and 38% threonine and serine and shows both N- and O-linked glycosylation. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Gene Symbol

SPN

UniProt

P16150

Additional CD43 Products

Product Documents for Human CD43 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD43 Biotinylated Antibody

For research use only

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FAQs

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