Human CD45RA Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD45RA

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA.  CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene.  In humans, CD45RA is one of six isoforms of the CD45 complex.  These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC.  CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells.  CD45RA contains exon 4 but lacks both exons 5 and 6.  CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons.  Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells.  Other cell types such as activated monocytes and B cells can also express CD45RO.  Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Product Datasheets

Related Research Areas

• Airway Inflammation and Remodeling
• Basophils
• Eosinophils
• Epigenetics
• Granulocyte Markers
• Mesenchymal Stem Cell Markers
• Monocyte Markers
• Negative Regulators of the Jak/STAT Pathway
• Neutrophils
• Osteoblast and Osteoclast Markers
• Phosphatases and Regulators
• Phosphatases in the Akt Pathway
• Regulatory T Cells (Tregs)
• Search Kinases and Phosphatases
• T Cell Antigen Recognition