Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 644730
View all EOMES Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936

Specificity

Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EOMES Antibody

Detection of Human EOMES antibody by Western Blot.

Detection of Human EOMES by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesendoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for EOMES at approximately 100 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EOMES antibody in Mesodermally Differentiated BG01V Human Stem Cells by Immunocytochemistry (ICC).

EOMES in Mesodermally Differentiated BG01V Human Stem Cells.

EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Monoclonal Antibody (Catalog # MAB6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of EOMES antibody in Differentiated BG01VHuman Cells antibody by Flow Cytometry.

Detection of EOMES in Differentiated BG01VHuman Cells by Flow Cytometry.

BG01V human embryonic stem cells differentiated to mesendoderm were stained with Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allo-phycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human EOMES Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into mesoderm

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: BG01V human embryonic stem cells differentiated to mesendoderm fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Mesendoderm differentiated BG01V human embryonic stem cells

Reviewed Applications

Read 4 reviews rated 4 using MAB6166 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EOMES

EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. 

Long Name

Eomesodermin Homolog

Alternate Names

Eomesodermin, TBR2

Entrez Gene IDs

8320 (Human); 13813 (Mouse); 316052 (Rat)

Gene Symbol

EOMES

UniProt

O95936 (Human)

Additional EOMES Products

Product Documents for Human EOMES Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EOMES Antibody

For research use only

Citations for Human EOMES Antibody

Customer Reviews for Human EOMES Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 的 4 reviews Showing All
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  • Human EOMES Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Embryonic stem cell derived mesoderm
    Species: Human
    Verified Customer | Posted 09/04/2021
    Human EOMES Antibody MAB6166
  • Human EOMES Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 03/30/2020
    EOMES on tonsil, 1:50
    Human EOMES Antibody MAB6166
  • Human EOMES Antibody
    Name: Olivier Etienne
    Application: Immunohistochemistry-Paraffin
    Sample Tested: embryonic mouse brain
    Species: Mouse
    Verified Customer | Posted 02/17/2020
    TBR2 in red, 1:300 DAPI in grey
    Human EOMES Antibody MAB6166
  • Human EOMES Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Embryonic stem cell derived mesoderm
    Species: Human
    Verified Customer | Posted 06/22/2016
    Human EOMES Antibody MAB6166

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Protocols

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