Human IGF-I/IGF-1 Antibody

R&D Systems | Catalog # AF-291-NA

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Bovine

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Immunoprecipitation, ELISA Development, ELISA Development (Detection), Neutralizing

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IGF-I/IGF-1

Specificity

Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IGF-I/IGF-1 Antibody

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.

Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3-12 µg/mL.
IGF-I/IGF-1 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC).

IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line.

IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGF-I/IGF-1 antibody in Human Placenta by Immunohistochemistry (IHC-P).

IGF-I/IGF-1 in Human Placenta.

IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human IGF-I/IGF-1 by Simple WesternTM.

Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Human IGF-I ELISA Standard Curve

Human IGF-I ELISA Standard Curve

Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IGF-I/IGF-1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Simple Western

50 µg/mL
Sample: Recombinant Human IGF-1

Western Blot

0.1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)

Neutralization

Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.

Reviewed Applications

Read 4 reviews rated 4.5 using AF-291-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for Human IGF-I/IGF-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IGF-I/IGF-1 Antibody

For research use only

Citations for Human IGF-I/IGF-1 Antibody

Customer Reviews for Human IGF-I/IGF-1 Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 4 的 4 reviews Showing All
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  • Human IGF-I/IGF-1 Antibody
    Name: Hannah Wickham
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Pancreas tissue
    Species: Canine
    Verified Customer | Posted 03/08/2022
    Used at 1:50 concentration in Casein in PBS overnight at 4 C. HIER was performed for two hours at 75 C in a citrate buffer.
    Human IGF-I/IGF-1 Antibody AF-291-NA
  • Human IGF-I Antibody
    Name: Alisha Freeman
    Application: Siteclcik antibody biotinylation
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 10/27/2017
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24036101
    Species: Human
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23424195
    Species: Human
    Verified Customer | Posted 01/07/2015

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