Human IL-8/CXCL8 Antibody

Catalog # Availability Size / Price Qty
AF-208-NA
AF-208-SP
Human IL-8/CXCL8 Antibody in Data
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Product Details
Citations (9)
FAQs
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Reviews (5)

Human IL-8/CXCL8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant canine
IL-8/CXCL8, recombinant feline IL-8/CXCL8 and recombinant porcine IL-8/CXCL8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL)
Simple Western
25 µg/mL
THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS and PBMC continioned media treated with LPS
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Simple Western View Larger

Detection of Human IL-8/CXCL8 by Simple WesternTM. Simple Western lane view shows PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Simple Western View Larger

Detection of Human IL-8/CXCL8 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hrs and 10ug/mL LPS for 3 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Neutralization Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. View Larger

Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND50is typically 0.1-0.5 µg/mL.

Immunocytochemistry IL-8/CXCL8 in Human PBMCs. View Larger

IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL-8/CXCL8 in Human Tonsil. View Larger

IL-8/CXCL8 in Human Tonsil. IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑208‑NA) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Citations for Human IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Cigarette smoking increases bronchial mucosal IL-17A expression in asthmatics, which acts in concert with environmental aeroallergens to engender neutrophilic inflammation
    Authors: LQ Siew, SY Wu, S Ying, CJ Corrigan
    Clin. Exp. Allergy, 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC
  2. Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line
    Authors: P Ortiz-Mont, A Londoño-Va, JP Vernot
    Cell Commun. Signal, 2017;15(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Necrotic cells influence migration and invasion of glioblastoma via NF-?B/AP-1-mediated IL-8 regulation
    Authors: SH Ahn, H Park, YH Ahn, S Kim, MS Cho, JL Kang, YH Choi
    Sci Rep, 2016;6(0):24552.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
    Authors: Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M
    J. Invest. Dermatol., 2009;129(3):650-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
    Authors: Bendrik C, Dabrosin C
    J. Immunol., 2009;182(1):371-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation.
    Authors: Hardison MT, Galin FS, Calderon CE, Djekic UV, Parker SB, Wille KM, Jackson PL, Oster RA, Young KR, Blalock JE, Gaggar A
    J. Immunol., 2009;182(7):4423-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development

FAQs

  1. Are mouse or rat CXCL8/IL-8 products offered?

    • Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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Reviews for Human IL-8/CXCL8 Antibody

Average Rating: 4.6 (Based on 5 Reviews)

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Human IL-8/CXCL8 Antibody
By Anonymous on 11/01/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). Paired with biotinylated MAB208 as a capture reagent. Calibration curve with Recombinant Human IL-8 (208-IL-010/CF) is shown (dynamic range 1.6-25,000 pg/ml).


Human IL-8/CXCL8 Antibody
By Anonymous on 04/05/2018
Application: ELISA Sample Tested: 293T human embryonic kidney cell line Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 01/18/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 10/09/2017
Application: PLA Sample Tested: 2120Ep human embryonal carcinoma cell line Species: Human

Always works well in our assay and reproducible from lot to lot. Would recommend!


Human CXCL8/IL-8 Antibody
By Anonymous on 09/09/2016
Application: IHC Sample Tested: Human breast tumor xenograft Species: Mouse

Antigen retrieval: 10mM Citrate pH6. Goat anti-IL-8 used at 1:500. IHC-DAB.