Human Integrin alpha V/CD51 Antibody (P2W7)

R&D Systems | Catalog # MAB1219

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Flow Cytometry, Bioassay, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P2W7
View all Integrin alpha V/CD51 Primary Antibodies »

Product Specifications

Immunogen

Ocular melanoma cell line

Specificity

Detects the ectodomain of human Integrin alpha V/CD51.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin alpha V/CD51 Antibody (P2W7)

Detection of Integrin aV/CD51 antibody in HT1080 Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).
Integrin aV/CD51 antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC).

Integrin alpha V/CD51 in HT1080 Human Cell Line.

Integrin aV/CD51 was detected in immersion fixed HT1080 human fibrosarcoma cell line using 10 µg/mL Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin alpha V/CD51 Antibody (P2W7)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HT1080 human fibrosarcoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB1219 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V/CD51

Integrin alpha V (CD51), also known as Vitronectin Receptor Subunit alpha is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 amino acid (aa) type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta ‑propellor domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

Product Documents for Human Integrin alpha V/CD51 Antibody (P2W7)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin alpha V/CD51 Antibody (P2W7)

For research use only

Citations for Human Integrin alpha V/CD51 Antibody (P2W7)

Customer Reviews for Human Integrin alpha V/CD51 Antibody (P2W7) (1)

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  • Human Integrin  alpha V/CD51 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HT1080 human fibrosarcoma cell line
    Species: Human
    Verified Customer | Posted 04/06/2022
    Human Integrin alpha V/CD51 Antibody (P2W7) MAB1219

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Protocols

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