Human Integrin beta 1/CD29 Antibody (P5D2)
R&D Systems | Catalog # MAB17781
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Papio cynocephalus (Yellow Baboon)
Applications
Validated:
Knockout Validated, Blockade of Receptor-ligand Interaction, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Bioassay, Blocking, ELISA Capture, Flow Cytometery
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P5D2
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Product Specifications
Immunogen
Human skin keratinocytes
Specificity
Detects human Integrin beta 1/CD29.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Integrin beta 1/CD29 Antibody (P5D2)
Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of Integrin beta 1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Integrin beta 1/CD29 in human PBMCs.
Integrin beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Integrin beta 1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line.
Integrin beta 1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog# F0102B). No staining in the Integrin beta 1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin beta 1/CD29 by Flow Cytometry
DC-conditioned medium inhibits mesenchymal stromal cells (MSC) differentiation into adipocytes through osteopontin release. (A) MSCs were cultured for 15 days in adipocyte differentiation medium in the presence of 30% DC-CM or RPMI (control condition, ctrl) and stained with Oil Red O to reveal lipid droplets (original magnification 5×) (left panel). Adipocytes were counted in five random fields from one representative well per group (middle panel) and Oil Red O extracted with isopropanol was measured at optical density 490 (right panel) (mean ± SEM of four independent wells). *p < 0.05 vs. ctrl by Student’s t-test. (B) The mRNA levels of ADIPOQ, FABP4, and PPAR gamma 2 were analyzed by real-time PCR at days 5 and 12 of culture. Data were shown as means ± SEM (n = 3). *p < 0.05 vs. ctrl by Student’s t-test. (C) MSCs were examined for the expression of CD29 and CD44 by flow cytometry (gray area, isotype control; white area, specific antibody). MSCs were induced by adipogenic differentiation medium in control condition, with rhOPN or DC-CM in the presence or the absence of the indicated antibodies. Relative mRNA expression of ADIPOQ and FABP4 was measured by real-time PCR on day 12 of adipogenic induction. RPL13A was used for normalization. Data were shown as means ± SEM (n = 3). *p < 0.05 vs. ctrl; #p < 0.05 vs DC-CM in presence of the isotype control by one-way ANOVA followed by Tukey’s Multiple Comparison Test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29910810), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Integrin beta 1/CD29 Antibody (P5D2)
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450.
Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and A549 human lung carcinoma cell line
Sample: Human peripheral blood mononuclear cells (PBMCs) and A549 human lung carcinoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Immunoprecipitation
Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
Joneckis, C.C. et al. (1993) Blood 82:3548.
Knockout Validated
Integrin beta 1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta 1/CD29 knockout A549 cell line.
Reviewed Applications
Read 5 reviews rated 4.6 using MAB17781 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Integrin beta 1/CD29
References
- Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
- Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
Additional Integrin beta 1/CD29 Products
Product Documents for Human Integrin beta 1/CD29 Antibody (P5D2)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Integrin beta 1/CD29 Antibody (P5D2)
For research use only
Citations for Human Integrin beta 1/CD29 Antibody (P5D2)
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Customer Reviews for Human Integrin beta 1/CD29 Antibody (P5D2) (5)
4.6 out of 5
5 Customer Ratings
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Application: Tethering reagent to attach non-adherent cellsSample Tested: Karpas 422 Human B cell non-Hodgkin lymphomaSpecies: HumanVerified Customer | Posted 07/19/2023Cell proliferation was measured by Xcelligence impedance assay.
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Application: Western BlotSample Tested: Tumor cell lysatesSpecies: HumanVerified Customer | Posted 03/20/2022
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Application: Western BlotSample Tested: Mucociliary epitheliumSpecies: HumanVerified Customer | Posted 04/02/2020
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Application: Western BlotSample Tested: Tumor cell lyastesSpecies: HumanVerified Customer | Posted 02/16/2020
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Application: Western BlotSample Tested: blood endothelial cellsSpecies: HumanVerified Customer | Posted 04/24/2018Worked in both reducing and non-reducing conditions Band seen around 130kDa Would use again on a new blot, not a stripped blot as got very similar band to that with alpha 5
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
