Human Laminin alpha 1 Antibody

Name: Human Laminin alpha 1 Antibody
Brand: R&D Systems
SKU: MAB4187
Rating: 5 (1 reviews)

Catalog #: MAB4187 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4187-100
MAB4187-SP

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Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Laminin alpha 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Laminin alpha 1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 775016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Laminin alpha 1
Leu22-Met269
Accession # P25391

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Laminin a1 antibody in U2OS Human Cell Line antibody by Flow Cytometry.

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Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry. U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Laminin a1 Monoclonal Antibody (Catalog # MAB4187, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry

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Laminin alpha 1 in U2OS Human Cell Line. Laminin a1 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and A549 human lung carcinoma cell line (negative stain) using Mouse Anti-Human Laminin a1 Monoclonal Antibody (Catalog # MAB4187) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 1

Laminin subunit alpha 1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha, beta, and gamma ) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha 1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha 1 shares 95% and 91% aa sequence identity with canine and mouse alpha 1 chain, respectively.

Entrez Gene IDs

284217 (Human); 16772 (Mouse); 316758 (Rat)

Alternate Names

LAMA; LAMA1 laminin, alpha 1 ; LAMA1; Laminin alpha 1; S-LAM-alpha