TREM2 Antibody (237920) [Biotin]

R&D Systems | Catalog # FAB17291B

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 237920
View all TREM2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM2
extracellular domain
Accession # Q99NH8

Specificity

Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for TREM2 Antibody (237920) [Biotin]

Detection of TREM2 in PBMCs with CD14 costain cells by Flow Cytometry.

PBMCs with CD14 costain were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Rat Anti-Human/Mouse TREM2 Biotinylated Monoclonal Antibody (Catalog # FAB17291B) or (B) Rat IgG2B Biotinylated Isotype Control (Catalog # IC013B) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Detection of TREM2 in RAW264 cells by Flow Cytometry.

RAW264 cells were stained with Rat Anti-Human/Mouse TREM2 Biotinylated Monoclonal Antibody (Catalog # FAB17291B, filled histogram) or isotype control antibody (Catalog # IC013B, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for TREM2 Antibody (237920) [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: PBMCs with CD14 costain and RAW 264.7 mouse monocyte/macrophage cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 0.2 μm filtered solution in RDF1.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

UniProt

Q99NH8 (Mouse)

Additional TREM2 Products

Product Documents for TREM2 Antibody (237920) [Biotin]

Certificate of Analysis

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Product Specific Notices for TREM2 Antibody (237920) [Biotin]

For research use only

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