Human Pro MMP-13 Antibody (87518)

R&D Systems | Catalog # MAB913

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation, Immunoaffinity Purification

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 87518
View all MMP-13 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452

Specificity

Detects human Pro MMP‑13 in direct ELISAs and Western blots. Does not detect active recombinant human MMP-13. In Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Pro MMP-13 Antibody (87518)

Human Pro- MMP-13 Antibody in ELISA Standard Curve.

Human Pro- MMP‑13 ELISA Standard Curve.

Recombinant Human Pro-MMP-13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP-13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro MMP-13 Monoclonal Antibody (Catalog # MAB913) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Pro MMP-13 Antibody (87518)

Application
Recommended Usage

ELISA

This antibody functions as a Pro-MMP-13 ELISA ELISA detection antibody when paired with Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-MMP-13 DuoSet ELISA Kit (Catalog # DY913) for convenient development of a sandwich ELISA or the Human Pro-MMP-13 Quantikine ELISA Kit (Catalog # DM1300) for a complete optimized ELISA.

Immunoaffinity Purification

Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human Pro-MMP-13 (Catalog # 511-MM) from condition media.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP-13 Western Blot Standard (Catalog # WBC020) under non‑reducing conditions only

Reviewed Applications

Read 1 review rated 4 using MAB913 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-13

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Entrez Gene IDs

4322 (Human)

Gene Symbol

MMP13

UniProt

P45452

Additional MMP-13 Products

Product Documents for Human Pro MMP-13 Antibody (87518)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pro MMP-13 Antibody (87518)

For research use only

Citations for Human Pro MMP-13 Antibody (87518)

Customer Reviews for Human Pro MMP-13 Antibody (87518) (1)

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  • Human Pro MMP-13 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: C20A4 Chondrocyte cell line
    Species: Human
    Verified Customer | Posted 08/28/2018
    Human Pro MMP-13 Antibody (87518) MAB913

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