Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 953728
View all TMIGD2/CD28H Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line-derived human TMIGD2/CD28H
Met1-Gly150
Accession # Q96BF3

Specificity

Detects human TMIGD2/CD28H in direct ELISAs. Stains human TMIGD2/CD28H transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TMIGD2/CD28H Antibody

Detection of TMIGD2/CD28H antibody in HEK293 Human Cell Line Transfected with Human CD28L/IGPR-1 and eGFP antibody by Flow Cytometry.

Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human CD28L/IGPR-1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human TMIGD2/CD28H or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB8316) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB004). View our protocol for Staining Membrane-associated Proteins.

TMIGD2/CD28H antibody in Human Thymus by Immunohistochemistry (IHC-P).

TMIGD2/CD28H in Human Thymus.

TMIGD2/CD28H was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB8316) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TMIGD2/CD28H Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thymus

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References

  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Alternate Names

CD28H, IGPR-1, IGPR1

Entrez Gene IDs

126259 (Human)

Gene Symbol

TMIGD2

UniProt

Q96BF3

Additional TMIGD2/CD28H Products

Product Documents for Human TMIGD2/CD28H Antibody

Certificate of Analysis

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Product Specific Notices for Human TMIGD2/CD28H Antibody

For research use only

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Protocols

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