Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse IgG F(ab')2 fragment

Specificity

No reaction was observed against Mouse IgG F(c).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Mouse IgG F(ab')2 Secondary Antibody

Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]
Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of paraffin embedded human colonic tissue. Image from verified customer review.
Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]
Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]

Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.
Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgG Secondary Antibody (Pre-absorbed) Conjugate at 1ug/mL blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the Cy5.5 version of this antibody.
Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng

Applications for Goat anti-Mouse IgG F(ab')2 Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Frozen

1:1000 - 1:5000

Western Blot

1:2
Application Notes
This product has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

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Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Goat anti-Mouse IgG F(ab')2 Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Mouse IgG F(ab')2 Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Mouse IgG F(ab')2 Secondary Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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