Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

Novus Biologicals | Catalog # NBP1-51900

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
View all IgG F(ab')2 Secondary Antibodies »

Product Specifications

Immunogen

Human IgG-F(ab')2 Fragment

Reactivity Notes

Minimum reactivity to mouse and rat.

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900]

Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900]

Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900] - Human cancer cell line. Image from verified customer review.

Applications for Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

Application
Recommended Usage

ELISA

1:1000 - 1:20000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:20000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. ELISA, for coating plates 1:50 - 1:250.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Format

Pre-adsorbed

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents for Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

Certificate of Analysis

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Product Specific Notices for Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed)

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Protocols

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FAQs

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