Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)

Novus Biologicals | Catalog # NB120-6253

Novus Biologicals

Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
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Product Specifications

Immunogen

Rat IgG F(c) fragment

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum

Scientific Data Images for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)

Dot Blot: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed) [NB120-6253] - Dot Blot results of Goat F(ab')2 anti-Rat IgG Fc Secondary antibody [FITC] (Pre-adsorbed).Dots are Rat F(c) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 60 min at RT.Primary Antibody: none.Secondary Antibody: Goat F(ab')2 Anti-Rat IgG Antibody FITC at 1ug/mL for 1hr at RT.Imaged with BioRad ChemiDoc, FITC filter.

Applications for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)

Application
Recommended Usage

Flow Cytometry

1:500 - 1:2500

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
This product has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)

Certificate of Analysis

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Product Specific Notices for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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