Armenian Hamster IgG Isotype Control (299Arm)

Name: Armenian Hamster IgG Isotype Control (299Arm)
Brand: Novus Biologicals
SKU: NBP2-59616
Price: 149 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-59616

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Product Documents

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Product Specifications
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Related Blogs

Key Product Details

Species Reactivity

Armenian Hamster

Applications

Immunohistochemistry, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Control, In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 299Arm

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Product Specifications

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Applications for Armenian Hamster IgG Isotype Control (299Arm)

Application

Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Application Notes

This product is useful as an isotype control immunoglobulin This product has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody..

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Armenian Hamster IgG Isotype Control (299Arm)

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Product Specific Notices for Armenian Hamster IgG Isotype Control (299Arm)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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