IgG1 Antibody (RM117)

Novus Biologicals | Catalog # NBP2-62028

Recombinant Monoclonal Antibody.
Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM117 expressed in HEK293
View all IgG1 Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to the hinge region of Human IgG1

Specificity

This antibody reacts to the heavy chain of human IgG1. It does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IgG1 Antibody (RM117) (NBP2-62028) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IgG1 Antibody (RM117)

IgG1 Antibody (RM117)

Sandwich ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] -

Sandwich ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] - Sandwich ELISA using RM117 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.
IgG1 Antibody (RM117)

ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] -

ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] - ELISA of human immunoglobulins shows RM117 only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG1 Antibody (RM117)

ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] -

ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP2-62028] - A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG1 Antibody (RM117)

Application
Recommended Usage

Flow Cytometry

2ug/ml-8ug/ml

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml

Immunohistochemistry-Paraffin

0.5ug/ml - 2ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for IgG1 Antibody (RM117)

Certificate of Analysis

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Product Specific Notices for IgG1 Antibody (RM117)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IgG1 Antibody (RM117)

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Protocols

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