IL-32 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76684
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
ELISA, Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32. The immunogen is located within the last 50 amino acids of IL-32. Amino Acid Sequence: YGAPRGDKEELTPQK
Specificity
This antibody detects all isoforms of IL-32. Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27 kDa), isoform 2 (188aa, 22 kDa), isoform 3 (178aa, 21 kDa), isoform 4 (131aa, 15 kDa), isoform 5 (168aa, 19 kDa), isoform 6 (179aa, 21 kDa) and isoform 7 (148aa, 17 kDa). NBP1-76684 can detect all human isoforms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit IL-32 Antibody - BSA Free (NBP1-76684) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-IL-32 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IL-32 Antibody - BSA Free
Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] -
Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, (Lane 1: 0.5 u/mL, Lane 2: 1 u/mL, Lane 3: 2 u/mL and Lane 4: 4 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.
Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] -
Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Validation of IL-32 in Human Spleen Tissue.Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: IL-32 Antibody - BSA Free [NBP1-76684] -
Immunocytochemistry/ Immunofluorescence: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 4 Immunofluorescence Validation of IL-32 in Human Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] -
Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 5 Immunohistochemistry Validation of IL-32 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-32 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] -
Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Validation in Human Spleen Lysate, showing two isoforms of IL-32Loading: 15 ug of lysates per lane.Antibodies: IL-32, (lane 1: 2 ug/mL and lane 2: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Applications for IL-32 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
2-10 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
0.5 - 4 ug/mL
Application Notes
Use in FLOW reported in scientific literature (PMID: 30641867).
Reviewed Applications
Read 1 review rated 2 using NBP1-76684 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-32
Long Name
Interleukin 32
Alternate Names
IL32, NK4, TAIF
Entrez Gene IDs
9235 (Human)
Gene Symbol
IL32
UniProt
Additional IL-32 Products
Product Documents for IL-32 Antibody - BSA Free
Product Specific Notices for IL-32 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for IL-32 Antibody - BSA Free
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Application: IHC, IFSample Tested: Human Tonsil tissueSpecies: HumanVerified Customer | Posted 07/27/2021Human Tonsil stained for IL32 (red) counterstained with DAPIPFA fixed sample
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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