Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Novus Biologicals | Catalog # NBP1-51541
![Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Western-Blot-NBP1-51541-img0012.jpg "Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5A11E9
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of Prostate Specific Antigen (amino acids 26-251) expressed in E. coli. [UniProt# P07288]
Localization
Secreted.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]
Western Blot: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis against truncated Prostate Specific Antigen recombinant protein.![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Immunohistochemistry-Paraffin-NBP1-51541-img0011.jpg "Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]")
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using Prostate Specific Antigen mouse mAb with DAB staining.![Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Flow-Cytometry-NBP1-51541-img0009.jpg "Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]")
Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]
Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Flow cytometric analysis of PC-3 cells using Prostate Specific Antigen mouse mAb (right) and negative control (left).![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]](https://resources.rndsystems.com/images/products/Kallikrein-3-PSA-Antibody-5A11E9-Immunohistochemistry-Paraffin-NBP1-51541-img0010.jpg "Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]")
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate tissues using Prostate Specific Antigen mouse mAb with DAB staining.Applications for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This Prostate Specific Antigen (5A11E9) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kallikrein 3/PSA
Additional Kallikrein 3/PSA Products
Product Documents for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
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Q: I am looking for a pair of antibodies against TOTAL PSA to be used in a sandwich immunoassay. I saw that you carry a biotiny-Ab (NBP1-44951). I was wondering if it can be used to detect TOTAL PSA and if you recommend a matched pair?
A:
A list of our PSA antibodies suitable for use in ELISA can be found using this link. None have been paired specifically with NBP1-44951 but any monoclonal on this list should suffice. I would recommend NBP1-51541 as it is our best selling antibody to this target and should be compatible with NBP1-44951.
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